Kinetics quality assessment for relative quantification by real-time PCR
نویسندگان
چکیده
منابع مشابه
Kinetics quality assessment for relative quantification by real-time PCR.
For proper relative quantification by real-time PCR, compared samples should have similar PCR efficiencies. To test this prerequisite, we developed two quality tests: (i) adjustment of a test for kinetic outlier detection (KOD) to relative quantification; and (ii) comparison of the efficiency variance of test samples with the efficiency variance of samples with highly reproducible quantificatio...
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Summary Chainy is a cross-platform web tool providing systematic pipelines and steady criteria to process real-time PCR data, including the calculation of efficiencies from raw data by kinetic methods, evaluation of the suitability of multiple references, standardized normalization using one or more references, and group-wise relative quantification statistical testing. We illustrate the utilit...
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ژورنال
عنوان ژورنال: BioTechniques
سال: 2005
ISSN: 0736-6205,1940-9818
DOI: 10.2144/05393st01